Open AccessDNA flow cytometric analysis of monogranulocytic colony forming cells from mouse bone marrow in Vitro
Author(s) -
Ronot X.,
Adolphe M.,
Hecquet C.,
Fontagne J.,
Jaffray P.,
Lechat P.
Publication year - 1983
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990030513
Subject(s) - propidium iodide , dna , microbiology and biotechnology , flow cytometry , in vitro , bone marrow , biology , cell cycle , staining , cell culture , cell , chemistry , immunology , biochemistry , genetics , apoptosis , programmed cell death
An adaptation of a previously reported flow cytometric technique is described. This technique was applied to study the DNA distribution of mouse granulocyte‐macrophage colony forming cells (GM‐CFc) grown in a methyl cellulose culture system. This method involved the collection of cell clusters and colonies from the methyl cellulose culture medium for the subsequent determination of DNA content. The DNA content of the homogeneous GM‐CFc suspensions was determined by applying a modification of the propidium iodide staining procedure described by Crissman et al . The relative percentage of cells in G 0 + G 1 , S, and G 2 + M stages of the cell cycle was calculated by applying mathematical analysis to the resulting DNA histograms. This method could be useful in studying the effects of drugs on GM‐CFc kinetics.