Open AccessFlow cytometry of human testicular tissue
Author(s) -
Pfitzer Peter,
Gilbert Peter,
Rölz Gero,
Vyska Karel
Publication year - 1982
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990030209
Subject(s) - flow cytometry , andrology , spermatogenesis , prophase , shock (circulatory) , biology , cirrhosis , meiosis , pathology , medicine , immunology , biochemistry , gene
Flow cytometry of testicular tissue from autopsies showed differences in the proportion of substained spermatozoa (1 C A ), spermatids (1 C B ), diploid 2C nuclei and premeiotic spermatozoa in the 4 C class. The evaluation of the histograms was performed with computer assistance. Healthy accident cases or those delivered in forensic medicine furnished the control values. Patients with hepatic cirrhosis showed a nearly complete loss of the haploid class, while cases with fatty degeneration of the liver demonstrated a reduction. Circulatory insufficiency in cases of acute shock resulted in an increase in the 4C values of meiotic prophase I and a decrease of spermatids. Spermatozoa were normal in these cases. After prolonged shock a nearly complete breakdown of spermatogenesis was observed. In cases of atherosclerosis an irregular degree of reduction was seen. Cancer patients showed a reduction of spermatogenesis in correlation to therapy.