Open AccessEMOSS: An epiillumination microscope objective slit‐scan flow system
Author(s) -
Weller Laura A.,
Wheeless Leon L.
Publication year - 1982
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990030105
Subject(s) - microscope , optics , fluorescence microscope , slit , fluorescence , materials science , microscopy , optical microscope , resolution (logic) , plane (geometry) , aperture (computer memory) , light sheet fluorescence microscopy , photomultiplier , flow (mathematics) , scanning electron microscope , physics , computer science , detector , geometry , mathematics , artificial intelligence , acoustics , mechanics
An epiillumination microscope objective slit‐scan flow system has been fabricated utilizing two dimensional slit scanning with hydrodynamic sample stream focussing. Low resolution (4 μm) analysis of cellular fluorescence is facilitated by the definition of a stabilized flow plane through hydrodynamic focussing. Coincidence of the region of stabilized flow with the focal plane of the microscope objective will allow for the collection and subsequent imaging of fluorescence from cells oriented along this plane. Two orthogonal slit‐scan contours are generated as a cell traverses the excitation region. It is hoped that the need for a three dimensional system will be precluded by preferential orientation of the cells in the region of stabilized flow. Cellular fluorescence is collected by a high numerical aperture epiillumination optical system and imaged onto two orthogonal slits. Two photomultiplier tubes are used to detect fluorescence. It is anticipated that the epiilumination microscope objective slit‐scan flow system will be used with a variety of fluorescent stains and markers, as well as extended to the research of light scattered by cells. (Steen, H. B., Cytometry 1:26–31, 1980).