Open AccessA microsample collection device for electrostatically sorted cells or particles and its preparative use for biochemical analysis
Author(s) -
Hinson W. G.,
Pipkin J. L.,
Hudson J. L.,
Anson J. F.,
Tyrer H.
Publication year - 1982
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020606
Subject(s) - isotachophoresis , isoelectric focusing , chromatography , sorting , resolution (logic) , sample preparation , sample (material) , polyacrylamide , computer science , chemistry , biology , microbiology and biotechnology , biochemistry , artificial intelligence , electrode , programming language , electrolyte , enzyme
Abstract Biochemical analysis of proteins extracted from mammalian cell nuclei which were sorted according to DNA content was greatly simplified using a special collection device to recover sorted nuclei. The device was developed for use with a sorting flow cytometer to provide sufficient numbers of particles to achieve adequate sensitivity and resolution while minimizing sample degradation and loss. The design, evaluation and application of the collection device were described with emphasis on sample preparation. Nuclear populations G 0 + G 1 , S, and G 2 + M, were sorted from regenerating rat liver tissue preparations and collected separately in the special chambers. Nuclear proteins were extracted and analyzed by polyacrylamide gel isoelectric focusing or capillary isotachophoresis.