Open AccessA standardized differential stain for hematology
Author(s) -
Lapen Daniel
Publication year - 1982
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020508
Subject(s) - stain , differential staining , staining , hematology analyzer , eosin , chromatography , chemistry , pathology , medicine
A stain composed of purified Azure B and Eosin Y has been developed for use in conjunction with an automated stainer. This system provides the consistency in chromatic properties required for automated blood‐cell analysis by pattern recognition systems. The stain represents a marked improvement in stability over those presently used with differential analyzers, but it is also an important advance toward a standardized stain for general hematological use. The purity of the component dyes, the stability of the final stain and the dependence of staining intensity on the stain/buffer ratio and dye concentration are discussed.