Open AccessComparative uptake of adriamycin and daunorubicin in sensitive and resistant friend leukemia cells measured by flow cytometry
Author(s) -
Tapiero H.,
Fourcade A.,
Vaigot P.,
Farhi J. J.
Publication year - 1982
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020506
Subject(s) - daunorubicin , sodium azide , flow cytometry , leukemia , cell , fluorescence , membrane , chemistry , cell membrane , microbiology and biotechnology , biophysics , biochemistry , biology , immunology , physics , quantum mechanics
Abstract Based on the fluorescence properties of adriamycin (ADM) and daunorubicin (DNR), uptake in sensitive and resistant Friend leukemia cells (FLC) was studied with the aid of the fluorescence activated cell sorter (FACS II). A quantitative cell by cell fluorescence intensity analysis showed a differential affinity of FLC to ADM and DNR. The cellular uptake of these two drugs was temperature dependent and was not hindered by sodium azide treatment; incorporation into isolated nuclei was not temperature dependent, nor hindered by sodium azide. Friend leukemia cell variants resistant to adriamycin (ADM‐RFLC) and to daunorubicin (DNR‐RFLC) were developed. The rate of uptake of ADM and DNR across the plasma membrane of these two cell variants was lower than in sensitive cells. Although these cells were crossresistant to both ADM and DNR, the drug‐induced fluorescence intensity was distributed differently in the corresponding resistant cell variants. We suggest therefore that resistance is the consequence of changes induced in the plasma membrane components. These changes may differ according to which drug is used.