Open AccessCalibration of flow cytometry detector systems
Author(s) -
Durand Ralph E.
Publication year - 1981
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020312
Subject(s) - photomultiplier , signal (programming language) , calibration , detector , fluorescence , intensity (physics) , amplifier , filter (signal processing) , optics , materials science , physics , optoelectronics , computer science , cmos , quantum mechanics , computer vision , programming language
A method of determining the relative intensity of the fluorescence signal measured by a fluorescence activated cell sorter is described. The calibration process is a multistep procedure, in which the signal gain due to the amplifier module is first evaluated, followed by determination of the photomultiplier response as a function of tube voltage for each fluorochrome and filter combination of interest. Using these values, a simple mathematical calculation then determines the relative intensity of a fluorescent signal, even for samples differing in intensity by several orders of magnitude.