Open AccessG2‐delay after irradiation with α‐particles as studied in synchronized cultures and by the bromodeoxyuridine‐33258H technique
Author(s) -
Hieber L.,
Beck H.P.,
LückeHuhle C.
Publication year - 1981
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020308
Subject(s) - irradiation , bromodeoxyuridine , cell cycle , cell division , cell culture , mitosis , cell , microbiology and biotechnology , biophysics , biology , andrology , chemistry , cell growth , physics , genetics , medicine , nuclear physics
Division delay of mouse L‐929 fibroblasts after α‐irradiation is due to a pronounced lengthening of their G2‐phase. Experiments on synchronously and asynchronously growing cultures revealed a cell cycle phasedependent sensitivity of this effect: Cells irradiated in G2 or at the Gl/S border suffered a longer G2‐delay than cells irradiated at mid‐ or late‐S. Progression through G2 was nearly normal at doses up to 0.3 Gy if cells were exposed during Gl phase.