Open AccessThe effect of hexamethylene‐bis‐acetamide on multiparameter analysis of friend leukemia cells
Author(s) -
Mishal Z.,
Fourcade A.,
Tapiero H.
Publication year - 1981
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020306
Subject(s) - acetamide , fluorescence , fluorescence anisotropy , chemistry , cell , leukemia , microbiology and biotechnology , cell culture , cell growth , biophysics , membrane , biology , biochemistry , immunology , genetics , physics , organic chemistry , quantum mechanics
Friend leukemia cells were induced to differentiate by hexamethylene‐bis‐acetamide (HMBA). The relationship between cell growth cell volume distribution, fluorescence intensity and fluorescence anisotropy of the membrane probe 1,6 diphenyl 1,3,5 hexatriene (DPH) was analyzed in differentiated and undifferentiated cells with the aid of the fluorescence activated cell sorter (FACS II). The induction of a differentiated state was associated with a decrease in cell volume and an increase of fluorescence anisotropy. The inhibition of this induction by dexamethazone prevented the decrease of cell volume without affecting the increase of fluorescence anisotropy. We conclude therefore that the high degree of anisotropy is not correlated to the differentiated state nor with the volume of the cells.