Open AccessThe application of flow cytometry for the quantification of the response of experimental tumors to irradiation
Author(s) -
Beck H.P.,
Brammer I.,
Zywietz F.,
Jung H.
Publication year - 1981
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990020110
Subject(s) - flow cytometry , cytometry , population , irradiation , biology , microbiology and biotechnology , chemistry , medicine , physics , environmental health , nuclear physics
Abstract R1H rhabdomyosarcomas of the rat were irradiated with 15 Gy of x‐rays and the depopulation and repopulation of the tumor was determined by means of flow cytometry, quantitative histology and colony assays. The results show that flow cytometry yields important information about the cell population kinetics of irradiated tumors, but misleading results are obtained if flow cytometry is not combined with the determination of the numerical density of cells.