Open AccessA model for the computer analysis of synchronous DNA distributions obtained by flow cytometry
Author(s) -
Fox Michael H.
Publication year - 1980
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990010114
Subject(s) - flow cytometry , cytometry , dna , flow (mathematics) , computer science , biological system , computational biology , chemistry , microbiology and biotechnology , biology , genetics , physics , mechanics
A new model for obtaining the distributions of cells in the cell cycle by computer analysis of flow cytometry data is proposed. The model is able to analyze distributions from both asynchronous and synchronous cell populations. A culture of HeLa cells is pulse‐labeled with tritiated thymidine at various times after sychronization by the mitotic selection method, then analyzed by flow cytometry and autoradiography. The results show good agreement between the percentage S‐phase cells determined by the model and by autoradiography.