Open AccessA novel cell surface antigen, 4C8, is expressed on human eosinophils
Author(s) -
Aoyama Yasutaka,
Yamane Takahisa,
Hino Masayuki,
Ohta Kensuke,
Suzuki Kenichi,
Kitagawa Seiichi,
Masuyama Junichi,
Tatsumi Noriyuki
Publication year - 2002
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.10047
Subject(s) - antigen , cd16 , flow cytometry , monoclonal antibody , microbiology and biotechnology , biology , antibody , immunology , eosinophil , blot , cd3 , cd8 , biochemistry , asthma , gene
Background: A novel monoclonal antibody, anti‐4C8, reacted with human peripheral lymphocytes and monocytes but not with neutrophils. In this study, we investigated whether the 4C8 antigen is expressed on human peripheral eosinophils. Methods: Expression of the 4C8 antigen on eosinophils was analyzed by flow cytometry and molecular analysis of the antigen was performed with eosinophils by Western blotting. Results: Among human peripheral granulocytes, the 4C8 antigen was expressed on CD16‐negative cells but not on CD16‐positive cells. The 4C8 antigen also appeared to be expressed on eosinophils. To confirm the latter finding, eosinophils were purified from peripheral blood. On flow cytometric analysis, anti‐4C8 antibody reacted with purified eosinophils. On Western blotting analysis, anti‐4C8 reacted with a single band of 80 kDa in lysates from purified eosinophils. The correlation between the percentage of eosinophils determined by May‐Giemsa staining and the percentage of 4C8‐positive/CD16‐negative cells among granulocytes was good ( r = 0.91, P < 0.0001). Conclusions: Only a few cell surface antigens are available to distinguish human peripheral eosinophils from neutrophils. The novel cell surface antigen, 4C8, is a useful new marker of human eosinophils. Cytometry (Clin. Cytometry) 50:8–13, 2002. © 2002 Wiley‐Liss, Inc.