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Non‐thermal plasma‐induced apoptosis in yeast Saccharomyces cerevisiae
Author(s) -
Čtvrtečková Lucie,
Pichová Alena,
Scholtz Vladimír,
Khun Josef,
Julák Jaroslav
Publication year - 2019
Publication title -
contributions to plasma physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.531
H-Index - 47
eISSN - 1521-3986
pISSN - 0863-1042
DOI - 10.1002/ctpp.201800064
Subject(s) - saccharomyces cerevisiae , apoptosis , reactive oxygen species , mutant , yeast , mitochondrion , microbiology and biotechnology , biology , mitochondrial ros , biochemistry , gene
The effect of non‐thermal plasma generated by the direct current (DC) corona discharge in the mode of transition spark is studied on a yeast Saccharomyces cerevisiae . The exposure to plasma increases production of reactive oxygen species (ROS) in cells, possibly causing the induction of apoptosis. To clarify the mechanism of apoptosis, its induction is tested not only on a wild strain of S. cerevisiae , but also on mutant strains: A deletion mutant Δyca1 without yeast metacaspase proves that in S. cerevisiae the apoptosis occurs partly by the caspase‐independent pathway. A petite strains with mutation in the mitochondria do not show pronounced ROS formation, but in spite of this, apoptosis is detected. Hence, mitochondrial ROS probably do not play an important role in induction of apoptosis.