Open AccessHuman‐engineered Treg‐like cells suppress FOXP3‐deficient T cells but preserve adaptive immune responses in vivo
Author(s) -
Sato Yohei,
Passerini Laura,
Piening Brian D,
Uyeda Molly Javier,
Goodwin Marianne,
Gregori Silvia,
Snyder Michael P,
Bertaina Alice,
Roncarolo MariaGrazia,
Bacchetta Rosa
Publication year - 2020
Publication title -
clinical and translational immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.321
H-Index - 34
ISSN - 2050-0068
DOI - 10.1002/cti2.1214
Subject(s) - foxp3 , immunology , immune system , biology , t cell , flow cytometry , adoptive cell transfer , interleukin 21 , il 2 receptor , cancer research , microbiology and biotechnology
Objectives Genetic or acquired defects in FOXP3 + regulatory T cells (Tregs) play a key role in many immune‐mediated diseases including immune dysregulation polyendocrinopathy, enteropathy, X‐linked (IPEX) syndrome. Previously, we demonstrated CD4 + T cells from healthy donors and IPEX patients can be converted into functional Treg‐like cells by lentiviral transfer of FOXP3 (CD4 LVFOXP3 ). These CD4 LVFOXP3 cells have potent regulatory function, suggesting their potential as an innovative therapeutic. Here, we present molecular and preclinical in vivo data supporting CD4 LVFOXP3 cell clinical progression. Methods The molecular characterisation of CD4 LVFOXP3 cells included flow cytometry, qPCR, RNA‐seq and TCR‐seq. The in vivo suppressive function of CD4 LVFOXP3 cells was assessed in xenograft‐versus‐host disease (xeno‐GvHD) and FOXP3‐deficient IPEX‐like humanised mouse models. The safety of CD4 LVFOXP3 cells was evaluated using peripheral blood (PB) humanised (hu)‐ mice testing their impact on immune response against pathogens, and immune surveillance against tumor antigens. Results We demonstrate that the conversion of CD4 + T cells to CD4 LVFOXP3 cells leads to specific transcriptional changes as compared to CD4 + T‐cell transduction in the absence of FOXP3, including upregulation of Treg‐related genes. Furthermore, we observe specific preservation of a polyclonal TCR repertoire during in vitro cell production. Both allogeneic and autologous CD4 LVFOXP3 cells protect from xeno‐GvHD after two sequential infusions of effector T cells. CD4 LVFOXP3 cells prevent hyper‐proliferation of CD4 + memory T cells in the FOXP3‐deficient IPEX‐like hu‐mice. CD4 LVFOXP3 cells do not impede in vivo expansion of antigen‐primed T cells or tumor clearance in the PB hu‐mice. Conclusion These data support the clinical readiness of CD4 LVFOXP3 cells to treat IPEX syndrome and other immune‐mediated diseases caused by insufficient or dysfunctional FOXP3 + Tregs.