Premium
Response‐Surface‐Optimized and Scaled‐Up Microbial Electrosynthesis of Chiral Alcohols
Author(s) -
Mayr Jeannine C.,
Rosa Luis F. M.,
Klinger Natalia,
Grosch JanHendrik,
Harnisch Falk,
Spiess Antje C.
Publication year - 2020
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201903428
Subject(s) - electrosynthesis , escherichia coli , chemistry , yield (engineering) , biocatalysis , alcohol dehydrogenase , electron transfer , faraday efficiency , plasmid , acetophenone , combinatorial chemistry , catalysis , biochemistry , enzyme , organic chemistry , materials science , reaction mechanism , electrochemistry , gene , electrode , metallurgy
A variety of enzymes can be easily incorporated and overexpressed within Escherichia coli cells by plasmids, making it an ideal chassis for bioelectrosynthesis. It has recently been demonstrated that microbial electrosynthesis (MES) of chiral alcohols is possible by using genetically modified E. coli with plasmid‐incorporated and overexpressed enzymes and methyl viologen as mediator for electron transfer. This model system, using NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis to convert acetophenone into ( R )‐1‐phenylethanol, is assessed by using a design of experiment (DoE) approach. Process optimization is achieved with a 2.4‐fold increased yield of 94±7 %, a 3.9‐fold increased reaction rate of 324±67 μ m h −1 , and a coulombic efficiency of up to 68±7 %, while maintaining an excellent enantioselectivity of >99 %. Subsequent scale‐up to 1 L by using electrobioreactors under batch and fed‐batch conditions increases the titer of ( R )‐1‐phenylethanol to 12.8±2.0 m m and paves the way to further develop E. coli into a universal chassis for MES in a standard biotechnological process environment.