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One‐Pot Enzyme Cascade for Controlled Synthesis of Furancarboxylic Acids from 5‐Hydroxymethylfurfural by H 2 O 2 Internal Recycling
Author(s) -
Jia HaoYu,
Zong MinHua,
Zheng GaoWei,
Li Ning
Publication year - 2019
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201902199
Subject(s) - chemistry , horseradish peroxidase , 5 hydroxymethylfurfural , galactose oxidase , organic chemistry , catalysis , oxidase test , biocatalysis , cofactor , alcohol , enzyme , reaction mechanism
Furancarboxylic acids are promising biobased building blocks in pharmaceutical and polymer industries. In this work, dual‐enzyme cascade systems composed of galactose oxidase (GOase) and alcohol dehydrogenases (ADHs) are constructed for controlled synthesis of 5‐formyl‐2‐furancarboxylic acid (FFCA) and 2,5‐furandicarboxylic acid (FDCA) from 5‐hydroxymethylfurfural (HMF), based on the catalytic promiscuity of ADHs. The byproduct H 2 O 2 , which is produced in GOase‐catalyzed oxidation of HMF to 2,5‐diformylfuran (DFF), is used for horseradish peroxidase (HRP)‐mediated regeneration of the oxidized nicotinamide cofactors for subsequent oxidation of DFF promoted by an ADH, thus implementing H 2 O 2 internal recycling. The desired products FFCA and FDCA are obtained with yields of more than 95 %.