Premium
Hydrogen‐Driven Cofactor Regeneration for Stereoselective Whole‐Cell C=C Bond Reduction in Cupriavidus necator
Author(s) -
AssilCompanioni Leen,
Schmidt Sandy,
Heidinger Petra,
Schwab Helmut,
Kourist Robert
Publication year - 2019
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201900327
Subject(s) - cupriavidus necator , chemistry , cofactor , stereoselectivity , hydrogen , regeneration (biology) , reduction (mathematics) , stereochemistry , combinatorial chemistry , catalysis , organic chemistry , enzyme , bacteria , biology , genetics , polyhydroxyalkanoates , geometry , mathematics , microbiology and biotechnology
The coupling of recombinantly expressed oxidoreductases to endogenous hydrogenases for cofactor recycling permits the omission of organic cosubstrates as sacrificial electron donors in whole‐cell biotransformations. This increases atom efficiency and simplifies the reaction. A recombinant ene‐reductase was expressed in the hydrogen‐oxidizing proteobacterium Cupriavidus necator H16. In hydrogen‐driven biotransformations, whole cells catalyzed asymmetric C=C bond reduction of unsaturated cyclic ketones with stereoselectivities up to >99 % enantiomeric excess. The use of hydrogen as a substrate for growth and cofactor regeneration is particularly attractive because it represents a strategy for improving atom efficiency and reducing side product formation associated with the recycling of organic cofactors.