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Efficient Bioconversion of Sucrose to High‐Value‐Added Glucaric Acid by In Vitro Metabolic Engineering
Author(s) -
Su HuiHui,
Guo ZeWang,
Wu XiaoLing,
Xu Pei,
Li Ning,
Zong MinHua,
Lou WenYong
Publication year - 2019
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201900185
Subject(s) - bioconversion , enzyme , in vitro , sucrose , metabolic engineering , nicotinamide adenine dinucleotide , chemistry , biochemistry , nad+ kinase , biocatalysis , raw material , cascade , combinatorial chemistry , organic chemistry , chromatography , fermentation , catalysis , reaction mechanism
Glucaric acid (GA) is a major value‐added chemicals feedstock and additive, especially in the food, cosmetics, and pharmaceutical industries. The increasing demand for GA is driving the search for a more efficient and less costly production pathway. In this study, a new in vitro multi‐enzyme cascade system was developed, which converts sucrose efficiently to GA in a single vessel. The in vitro system, which does not require adenosine triphosphate (ATP) or nicotinamide adenine dinucleotide (NAD + ) supplementation, contains seven enzymes. All enzymes were chosen from the BRENDA and NCBI databases and were expressed efficiently in Escherichia coli BL21(DE3). All seven enzymes were combined in an in vitro cascade system, and the reaction conditions were optimized. Under the optimized conditions, the in vitro seven‐enzyme cascade system converted 50 m m sucrose to 34.8 m m GA with high efficiency (75 % of the theoretical yield). This system represents an alternative pathway for more efficient and less costly production of GA, which could be adapted for the synthesis of other value‐added chemicals.