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The Accessible Cellulose Surface Influences Cellulase Synergism during the Hydrolysis of Lignocellulosic Substrates
Author(s) -
Hu Jinguang,
Gourlay Keith,
Arantes Valdeir,
Van Dyk J. S.,
Pribowo Amadeus,
Saddler Jack N.
Publication year - 2015
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201403335
Subject(s) - cellulase , cellulose , cellulosic ethanol , chemistry , hydrolysis , substrate (aquarium) , enzymatic hydrolysis , cellulosome , beta glucosidase , organic chemistry , biochemistry , clostridium thermocellum , biology , ecology
Effective enzymatic hydrolysis of insoluble cellulose requires the synergistic action of a suite of cellulase components. Most previous studies have only assessed cellulase synergism on model cellulosic substrates. When the actions of individual and combinations of cellulases (Cel7A, Cel6A, Cel7B, Cel5A) were assessed on various pretreated lignocellulosic substrates, Cel7A was shown to be the major contributor to overall cellulose hydrolysis, with the other enzymes synergistically enhancing its hydrolytic efficiency, at least partially, by facilitating Cel7A desorption (assessed by a double‐sandwich enzyme‐linked immunosorbent assay). When the influences of various substrate physicochemical characteristics on the effectiveness of enzyme synergism were assessed, a strong relationship was observed between cellulose accessibility (as determined by the cellulose binding module technique) and the degree of synergism, with greater synergy observed on the more disorganized/accessible cellulose surface.