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Recycling of Carbon Dioxide and Acetate as Lactic Acid by the Hydrogen‐Producing Bacterium Thermotoga neapolitana
Author(s) -
d'Ippolito Giuliana,
Dipasquale Laura,
Fontana Angelo
Publication year - 2014
Publication title -
chemsuschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.412
H-Index - 157
eISSN - 1864-564X
pISSN - 1864-5631
DOI - 10.1002/cssc.201402155
Subject(s) - carbon dioxide , chemistry , fermentation , lactic acid , carboxylation , ferredoxin , mixed acid fermentation , hydrogen , biomass (ecology) , bacteria , carbon fixation , catalysis , lactic acid fermentation , organic chemistry , biochemistry , enzyme , biology , agronomy , genetics
The heterotrophic bacterium Thermotoga neapolitana produces hydrogen by fermentation of sugars. Under capnophilic (carbon dioxide requiring) conditions, the process is preferentially associated with the production of lactic acid, which, as shown herein, is synthesized by reductive carboxylation of acetyl coenzyme A. The enzymatic coupling is dependent on the carbon dioxide stimulated activity of heterotetrameric pyruvate:ferredoxin oxidoreductase. Under the same culture conditions, T. neapolitana also operates the unfavorable synthesis of lactic acid from an exogenous acetate supply. This process, which requires carbon dioxide (or carbonate) and an unknown electron donor, allows for the conversion of carbon dioxide into added‐value chemicals without biomass deconstruction.