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Enhancing oleic acid content in two commercially released peanut varieties through marker‐assisted backcross breeding
Author(s) -
Jadhav Mangesh Pralhad,
Patil Malagouda D.,
Hampannavar Mahesh,
Dattatreya Pavana,
Shirasawa Kenta,
Pasupuleti Janila,
Pandey Manish K.,
Varshney Rajeev K.,
Bhat Ramesh S.
Publication year - 2021
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.1002/csc2.20512
Subject(s) - backcrossing , biology , marker assisted selection , rust (programming language) , allele , cultivar , arachis hypogaea , genetic marker , horticulture , oleic acid , mutant , genetics , botany , gene , computer science , programming language
Two elite cultivars of peanut ( Arachis hypogaea L.), GPBD 4 and G 2–52, with high productivity, oil content, resistance to late leaf spot [ Phaeoisariopsis personata (Berk. & Curt) V. Arx.] (LLS) and rust ( Puccinia arachidis Speg.) diseases were improved for oleic acid content using marker‐assisted backcrossing. Since both the recurrent parents already possessed the mutant allele at AhFAD2A , only mutant allele at AhFAD2B was transferred from the donor ‘SunOleic 95R’ (oleate of 80.6%). Three rounds of backcrossing with foreground selection using allele‐specific polymerase chain reaction (PCR) and Kompetitive allele‐specific PCR (KASP) assay identified a large number of plants homozygous for the mutant allele at AhFAD2B in BC n F 2 generations. Evaluation of the advanced generations could identify six and 10 lines with significantly higher oleate than GPBD 4 and G 2–52, respectively. Considering the yield, shelling percentage, oil, and oleate content, the most promising lines HOBC 2 GPS_7 and HOBC 2 G2S_5 were selected with 112 and 142% oleate recovery over GPBD 4 and G 2–52, respectively. Double digest restriction‐site‐associated DNA sequencing of these superior lines showed background genome recovery of 77.5 and 69.0%, respectively. These advanced breeding lines with high oleate (∼80%), resistance to LLS and rust and high productivity are under further trials for possible release as varieties for commercial cultivation.