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Genome‐wide association analysis of resistance to aluminum toxicity in the roots of common bean seedlings
Author(s) -
Siamasonta Farai,
Njobvu Justine,
Hamabwe Swivia M.,
Munyinda Kalaluka,
Kamfwa Kelvin
Publication year - 2021
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.1002/csc2.20454
Subject(s) - biology , quantitative trait locus , single nucleotide polymorphism , seedling , phaseolus , genotype , genetics , horticulture , genetic variation , gene , botany
Abstract Aluminum toxicity is a major production constraint of common bean ( Phaseolus vulgaris L.). Here, we conducted a genome‐wide association study with an Andean diversity panel comprising 411 genotypes to identify genotypes with superior seedling Al resistance, and detect genomic regions associated with Al resistance in the Andean gene pool. The Andean diversity panel was evaluated for seedling Al resistance in a screen house with a hydroponic system with two nutrient solutions containing 0 μM (control solution) or 15 μM Al (Al stress solution). Seedling root length (RL) and root dry weight (RDW) were measured. Percentage reductions in RL (RL%) and in RDW (RDW%)were calculated as indices for Al resistance. Genome‐wide association analyses were conducted with 24,772 single nucleotide polymorphism (SNP) markers. Three genotypes [‘Msafiri’ (ADP70), ‘G5686’ (ADP566), and ‘Drake’ (ADP623)] showed high seedling Al resistance among the 411 genotypes. These genotypes could be used as sources of Al resistance to develop Al‐resistant varieties. Single nucleotide polymorphisms significantly associated with Al resistance were identified on chromosomes Pv 01, Pv 02, Pv 03, Pv 05, Pv 06, and Pv 10. Phenotypic variation explained by significant SNPs ranged from 7.1 to 21.1%, suggesting that genetic control for Al resistance in the Andean gene pool is under both minor‐ and major‐effect quantitative trait loci (QTLs) with additive action. The most significant SNP on Pv 02, which explained 21.1% of the phenotypic variation, overlapped with two previously reported QTLs for Al resistance. This major‐effect QTL on Pv 02 is a strong candidate for use in marker‐assisted selection to accelerate the development of Al‐resistant bean varieties.