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Characterization of post‐haustorial resistance to sunflower broomrape
Author(s) -
MartínSanz Alberto,
PérezVich Begoña,
Rueda Sandra,
FernándezMartínez José M.,
Velasco Leonardo
Publication year - 2020
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.1002/csc2.20002
Subject(s) - biology , orobanche , helianthus annuus , sunflower , parasitic plant , population , helianthus , genetics , inbred strain , gene , botany , host (biology) , horticulture , demography , sociology
The development of durable resistance to broomrape ( Orobanche cumana Wallr.) in sunflower ( Helianthus annuus L.) requires detailed characterization of the genetic and physiological bases of resistance. The objective of the present study was to map the resistance gene accurately, and to characterize the mechanism of resistance to broomrape observed in a sunflower inbred line (PHSC1102). PHSC1102, which was consistently resistant against race F and race G populations of broomrape, was crossed with PHSC1201, which was susceptible to races F and G. A mapping population of 150 F 2 genotypes was phenotyped by evaluating F 2:3 families for resistance to broomrape races F GV and G TK . The use of single nucleotide polymorphism (SNP) markers mapped the Or SII gene to Linkage Group 4 (LG4) of the sunflower genome. Mini‐rhizotron and histological studies of the resistant line revealed that the attachment of broomrape to host roots was similar in both the resistant and susceptible lines and that the resistance was observed at a late stage (i.e., after tubercle development). Interestingly, the resistance of the PHSC1102 line was associated with the production of phenolic compounds, which were hypothesized to restrict the parasite's growth. This research provides novel and valuable information about the host–parasite interactions between sunflower and broomrape.