Open AccessEffects of advanced platelet‐rich fibrin combined with xenogenic bone on human periodontal ligament stem cells
Author(s) -
Nguyen Meo,
Nguyen Thuy Thu,
Tran Ha Le Bao,
Tran Dang Ngoc,
Ngo Lan Thi Quynh,
Huynh Nam CongNhat
Publication year - 2022
Publication title -
clinical and experimental dental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.464
H-Index - 9
ISSN - 2057-4347
DOI - 10.1002/cre2.563
Subject(s) - platelet rich fibrin , periodontal ligament stem cells , fibrin , periodontal fiber , chemistry , cell growth , growth factor , exudate , platelet derived growth factor receptor , vascular endothelial growth factor , cell migration , andrology , cell , stem cell , microbiology and biotechnology , dentistry , immunology , vegf receptors , alkaline phosphatase , biology , pathology , cancer research , medicine , biochemistry , enzyme , receptor
Objectives In this study, we aimed to investigate the effects of a mixture of advanced platelet‐rich fibrin (A‐PRF) and xenogenic bone substitute material (XBSM) on the proliferation and migration of human periodontal ligament stem cells (hPDLSCs) based on the in vitro release of growth factors. Material and Methods The concentrations of platelet‐derived growth factor‐AB (PDGF‐AB) and vascular endothelial growth factor (VEGF) released by the A‐PRF‐XBSM mixture were estimated using enzyme‐linked immunoassay for up to 7 d. The A‐PRF‐XBSM mixture exudate was incubated with hPDLSCs. At Days 1, 3, 5, and 7, cell proliferation and migration were investigated by cell counting and wound‐healing assays. Results PDGD‐AB and VEGF were released from the A‐PRF‐XBSM mixture exudate for up to 7 days. hPDLSCs were cultured in media with various concentrations of the A‐PRF‐XBSM mixture exudate and exhibited their proliferation and migration ability. Furthermore, the factors released from the 100% A‐PRF‐XBSM mixture exudate had a substantial effect on cell migration, whereas those released from 4% and 20% A‐PRF‐XBSM mixture exudates stimulated hPDLSC proliferation. Conclusions A‐PRF‐XBSM mixture continuously released growth factors over 7 days and enhanced hPDLSC proliferation and migration. Therefore, A‐PRF in combination with XBSM might provide potential advantages for periodontal tissue regeneration.