Open AccessAnalysis of DNA methylation of E‐cadherin and p16 ink4a in oral lichen planus/oral lichenoid lesions
Author(s) -
Chujo Takatoshi,
Yoshida Koki,
Takai Rie,
Uehara Osamu,
Matsuoka Hirofumi,
Morikawa Tetsuro,
Sato Jun,
Chiba Itsuo,
Matsuzaka Kenichi,
Abiko Yoshihiro
Publication year - 2021
Publication title -
clinical and experimental dental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.464
H-Index - 9
ISSN - 2057-4347
DOI - 10.1002/cre2.355
Subject(s) - dna methylation , methylation , epigenetics , oral lichen planus , polymerase chain reaction , immunohistochemistry , biology , pathology , microbiology and biotechnology , cancer research , dna , biopsy , pathogenesis , bisulfite sequencing , gene , medicine , gene expression , genetics
Objectives Epigenetic phenomena are changes in gene expression not involving the DNA sequence. DNA methylation is a major occurrence underlying epigenetic changes in human cells. Although aberrant DNA methylation is well documented in malignant lesions, limited information has been shown on the involvement of DNA methylation in oral lichen planus and oral lichenoid lesions (OLP). The present study aimed to investigate DNA methylation of E‐cadherin and p16 in OLP, and compare the findings with those in non‐inflamed gingiva (Non), radicular cyst (RC), and oral squamous cell carcinoma (SCC). Materials and methods Paraffin‐embedded surgical biopsy specimens were sliced, DNA was extracted, bisulfite treatment was applied, and methylation‐specific polymerase chain reaction was performed. Immunohistochemistry was performed to observe the relative expression patterns of these genes. Results E‐cadherin was hypermethylated in OLP ( p < 0.01), SCC ( p < 0.01), and RC ( p < 0.05), when compared with Non; DNA hypermethylation was confirmed in OLP and SCC when compared to Non and RC. Hypermethylation of p16 ink4a was observed only in SCC ( p < 0.01). Conclusion DNA methylation levels of E‐cadherin and p16 ink4a were significantly higher in OLP than in normal tissues, and may be associated with the pathogenesis and progression of the disease.