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A novel “top‐down” strategy for preparing organosilica micelle encapsulating multiple hydrophobic quantum dots as efficient fluorescent label
Author(s) -
Liu Hongxin,
Liang Yuan,
Li Nana,
Wu Gang
Publication year - 2017
Publication title -
crystal research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.377
H-Index - 64
eISSN - 1521-4079
pISSN - 0232-1300
DOI - 10.1002/crat.201600328
Subject(s) - micelle , fluorescence , microemulsion , sonication , sodium silicate , chemical engineering , quantum dot , nanodiamond , pulmonary surfactant , chemistry , nanoparticle , materials science , amorphous solid , silane , sodium dodecyl sulfate , thioglycolic acid , nanotechnology , organic chemistry , chromatography , aqueous solution , physics , quantum mechanics , diamond , engineering
Organosilica micelle incorporating multiple hydrophobic CdSe/ZnS quantum dots (QDs) was prepared by a surfactant free microemulsion approach assisted by ultrasonication. The size of emulsion droplets along with their QDs loading amount was controlled and determined by QDs concentration in alkylsilane. Sodium silicate deposition was important in transforming the amorphous micelles into spherical beads with well defined QDs/SiO 2 structure. These QD micelles were subsequently modified with epoxy group by silane coupling and covalently conjugated to transferrin. After incubated with Hela cells, these fluorescent micelles were detected as bright clusters in cells, which demonstrated the efficient cell internalization of these functionalized particles.