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The crystallization of lysozyme in the system of ionic liquid [BMIm][BF 4 ]‐water
Author(s) -
Li Xinxin,
Xu Xiaodong,
Dan Yuanyuan,
Feng Jing,
Ge Lan,
Zhang Milin
Publication year - 2008
Publication title -
crystal research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.377
H-Index - 64
eISSN - 1521-4079
pISSN - 0232-1300
DOI - 10.1002/crat.200800040
Subject(s) - lysozyme , crystallization , ionic liquid , tetrafluoroborate , chemistry , crystallography , crystal growth , kinetics , chemical engineering , organic chemistry , catalysis , physics , biochemistry , quantum mechanics , engineering
Lysozyme crystallization was conducted in the ionic liquid (IL) 1‐butyl‐3‐methylimidizolium tetrafluoroborate ([BMIm][BF 4 ]) with different buffer/IL proportions. It was found that the addition of [BMIm][BF 4 ] could promote the crystallization process, during which more lager single crystals with controllable morphologies could be obtained due to the manageable crystal growth velocity. A probable explanation was proposed based on the influence of the ionic polarization and kinetics in the lysozyme crystallization. Moreover, the transform in coordination number and the relative growth rate of different crystal faces were discussed. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)