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Study of the crystal shape and its influence on the anti‐tumor activity of tumor necrosis factor‐related apoptosis‐inducing ligand (Apo2L/TRAIL)
Author(s) -
Yin JiCheng,
Zhou JinSong,
Sun Jing,
Qiu Yi,
Wei DongZhi,
Shen YaLing
Publication year - 2008
Publication title -
crystal research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.377
H-Index - 64
eISSN - 1521-4079
pISSN - 0232-1300
DOI - 10.1002/crat.200711135
Subject(s) - trimer , crystallization , apoptosis , ligand (biochemistry) , suspension (topology) , chemistry , tumor necrosis factor alpha , hexagonal crystal system , necrosis , crystal (programming language) , biophysics , crystallography , biochemistry , receptor , biology , organic chemistry , immunology , dimer , computer science , medicine , mathematics , homotopy , pure mathematics , programming language
This paper describes a method about the crystal shape control improving the anti‐tumor activity of tumor necrosis factor‐related apoptosis‐inducing ligand (Apo2L/TRAIL) in a batch cooling suspension crystallization by selecting pH values as a controllable variable. Three shaped TRAIL crystals could be obtained under different pH conditions, among which the hexagonal plate crystals had the highest specific activities against tumor cell line. The relationship among pH values, trimer contents and the specific activities of crystals and the purified TRAIL solutions were investigated. The results showed that different trimer contents resulted from pH altering in crystals and protein solutions is a main reason for their different specific activities. The studies may supply a new method to improve the bioactivity of TRAIL agent during its production and storage. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)