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Measurement of temperature and concentration dependences of refractive index of hen‐egg‐white lysozyme solution
Author(s) -
Yin D. C.,
Inatomi Y.,
Wakayama N. I.,
Huang W. D.
Publication year - 2003
Publication title -
crystal research and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.377
H-Index - 64
eISSN - 1521-4079
pISSN - 0232-1300
DOI - 10.1002/crat.200310096
Subject(s) - refractive index , lysozyme , wavelength , egg white , chemistry , interferometry , dissolution , quartz , analytical chemistry (journal) , optics , materials science , chromatography , optoelectronics , composite material , organic chemistry , biochemistry , physics
This paper reports the measurement of temperature and concentration dependences of the refractive index of hen‐egg‐white lysozyme (HEWL) solution, and the temperature dependence of refractive index of the quartz container, which are indispensable for the efforts to measure HEWL concentration of the solution during the processes like crystal growth and dissolution using interferometer. As all of these dependences might be different under different observation wavelengths, temperatures and solution compositions, it is necessary to measure them for specific conditions. In this paper, the desired dependences were measured without knowing the actual value of the refractive index. The measurement method was introduced and the measurement experiments were undertaken at wavelength 780 nm. The dependences obtained will be also useful for those studies employing the same wavelength and solution conditions. (© 2003 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)