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Immunopurification of Mitochondria from Arabidopsis
Author(s) -
Luo Lilan,
He Yajun,
Li Jiayang,
Yu Hong
Publication year - 2021
Publication title -
current protocols
Language(s) - English
Resource type - Journals
ISSN - 2691-1299
DOI - 10.1002/cpz1.34
Subject(s) - chloroplast , mitochondrion , organelle , arabidopsis , microbiology and biotechnology , biology , computational biology , chemistry , biochemistry , gene , mutant
Isolation of mitochondria from plant tissues, especially green leaves, is particularly difficult because of chloroplast contamination. Compared to other techniques for purifying plant mitochondria, the immunopurification method has a number of advantages: it rapidly purifies the mitochondria within several minutes, avoids loss of mitochondrial metabolites, eliminates most other organelles (especially the chloroplasts), keeps the isolated mitochondria intact, requires little starting material, and has the potential to purify tissue‐specific mitochondria. Here, we describe a rapid immunopurification protocol for mitochondrial isolation that employs a protein specifically targeted to the outer mitochondrial membrane that acts as an immunopurification handle. © 2021 Wiley Periodicals LLC. This article was corrected on 19 July 2022. See the end of the full text for details.