Premium
Overview of Methods for Purification and Characterization of Metalloproteins
Author(s) -
Harnden Kevin A.,
Roy Anindya,
Hosseinzadeh Parisa
Publication year - 2021
Publication title -
current protocols
Language(s) - English
Resource type - Journals
ISSN - 2691-1299
DOI - 10.1002/cpz1.234
Subject(s) - metalloprotein , characterization (materials science) , metal ions in aqueous solution , chemistry , redox , metal , nanotechnology , biochemical engineering , combinatorial chemistry , materials science , inorganic chemistry , engineering , organic chemistry
Metalloproteins make up one third of all proteins and perform some of the most essential reactions on earth. The unique properties of the metal ions within these proteins, and in particular of redox‐active metal ions, enables the use of a number of characterization techniques. It also necessitates unique considerations in terms of purification and characterization. In this overview, we describe the considerations and methods used for metalloprotein purification and characterization. © 2021 Wiley Periodicals LLC.