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Platelet Immunophenotyping by High‐Dimensional Mass Cytometry
Author(s) -
Spurgeon Benjamin E. J.,
Michelson Alan D.,
Frelinger Andrew L.
Publication year - 2021
Publication title -
current protocols
Language(s) - English
Resource type - Journals
ISSN - 2691-1299
DOI - 10.1002/cpz1.112
Subject(s) - immunophenotyping , mass cytometry , flow cytometry , platelet , cytometry , medicine , immunology , biology , phenotype , genetics , gene
Platelets are small blood cells that contribute to hemostasis, immunity, and inflammation. Characterization of platelet surface markers allows for differentiation of activated platelets from resting platelets, diagnosis of platelet disorders, and investigation of platelet biology and pathology. In this article, we describe the use of mass cytometry or “CyTOF” (mass spectroscopy detection of metal‐tagged antibodies on individual cells) to measure a large number of markers on each platelet and to identify platelet subsets based on the shared expression of multiple markers. This powerful new approach provides a vastly more detailed picture of platelet immunophenotypes than conventional flow cytometry and enables investigation of the roles of platelet subsets in health and disease. © 2021 Wiley Periodicals LLC. This article was corrected on 28 July 2022. See the end of the full text for details. Basic Protocol : Platelet immunophenotyping by high‐dimensional mass cytometry Support Protocol : Data preprocessing