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Preparation of Artificial Hexaplex Composed of Non‐Natural Nucleic Acid
Author(s) -
Makino Koki,
Hattori Yuhei,
Kashida Hiromu,
Asanuma Hiroyuki
Publication year - 2021
Publication title -
current protocols
Language(s) - English
Resource type - Journals
ISSN - 2691-1299
DOI - 10.1002/cpz1.106
Subject(s) - moiety , nucleic acid , tethering , nucleobase , cyanuric acid , combinatorial chemistry , chemistry , supramolecular chemistry , oligomer , stereochemistry , biochemistry , biology , molecule , organic chemistry , dna , melamine , microbiology and biotechnology
Abstract This article describes synthetic procedures for acyclic D ‐threoninol nucleic acid tethering of bifacial nucleobases. Because these nucleobases have complementary hydrogen bonding sites on both sides, their oligomers can form a hexaplex. These hexaplexes are suitable for use as metal or pH sensors and as supramolecular motifs. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Synthesis of D ‐threoninol backbone Basic Protocol 2 : Synthesis of D ‐ a TNA tethering cyanuric acid Basic Protocol 3 : Synthesis of D ‐ a TNA tethering a 2‐aminopyrimidine moiety Basic Protocol 4 : Synthesis of D ‐ a TNA tethering a 2,4,6‐triaminopyrimidine moiety Basic Protocol 5 : Synthesis of D ‐ a TNA oligomer tethering cyanuric acid, 2‐aminopyrimidine, or 2,4,6‐triaminopyrimidine