Preparation of Artificial Hexaplex Composed of Non‐Natural Nucleic Acid

This article describes synthetic procedures for acyclic D ‐threoninol nucleic acid tethering of bifacial nucleobases. Because these nucleobases have complementary hydrogen bonding sites on both sides, their oligomers can form a hexaplex. These hexaplexes are suitable for use as metal or pH sensors and as supramolecular motifs. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Synthesis of D ‐threoninol backbone Basic Protocol 2 : Synthesis of D ‐ a TNA tethering cyanuric acid Basic Protocol 3 : Synthesis of D ‐ a TNA tethering a 2‐aminopyrimidine moiety Basic Protocol 4 : Synthesis of D ‐ a TNA tethering a 2,4,6‐triaminopyrimidine moiety Basic Protocol 5 : Synthesis of D ‐ a TNA oligomer tethering cyanuric acid, 2‐aminopyrimidine, or 2,4,6‐triaminopyrimidine