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Development of a radioimmunoassay for theophylline; Application to studies in premature infants
Author(s) -
Neese Allen L.,
Soyka Lester F.
Publication year - 1977
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1002/cpt1977215633
Subject(s) - theophylline , radioimmunoassay , aminophylline , titer , antiserum , chemistry , chromatography , medicine , endocrinology , antibody , immunology
The use of theophylline in premature infants has necessitated the development of an assay applicable to samples under 0.1 ml. To accomplish this we have developed a radioimmunoassay for theophylline. The antiserum to theophylline has been characterized and found to be highly specific for theophylline and sensitive to 2 ng. The affinity of our antibody and the titer developed in the goat allowed the assay to be performed routinely with the use of a plasma sample size of only 2 to 5 µl. Theophylline levels of 6 to 20 µg/ml were found 2 hr after maintenance doses of aminophylline, 4 mg/kg per rectum in 12 premature infants. Terminal serum half‐lives ranged from 12 to 54 hr.