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Antipyrine: Radioimmunoassay in plasma and saliva following administration of a high dose and a low dose
Author(s) -
Chang R. L.,
Wood A. W.,
Dixon W. R.,
Conney A. H.,
Anderson K. E.,
Eiseman J.,
Alvares A. P.
Publication year - 1976
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1002/cpt1976202219
Subject(s) - radioimmunoassay , saliva , chemistry , chromatography , immunogen , medicine , plasma levels , plasma concentration , endocrinology , antibody , biochemistry , immunology , monoclonal antibody
A simple and sensitive radioimmunoassay has been developed for the determination of antipyrine levels in plasma and salim of man. Antiserum to antipyrine was obtained from rabbits immunized with an immunogen prepared by covalently coupling N‐(4‐antipyrinyl)‐succinamic acid to bovine serum albumin (BSA). The radioimmunoassay can detect antipyrine levels as low as 10 ng/ml of plasma or saliva, using a 0.1‐ml sample. This contrasts with the sensitivity of a commonly used spectrophotometric method that can measure about 4,000 ng/ml using a 2‐ml plasma sample. Agreement between the radioimmunoassay and spectrophotometric assay of antipyrine was excellent for plasma (r = 0.98) and saliva (r = 0.97) when samples were analyzed from 6 subjects receiving 18 mg/kg of antipyrine. The correlation between plasma and saliva antipyrine half‐lives using the radioimmunoassay and an 18 mg/kg dose of antipyrine was r = 0.90 (p < 0.005). After a dose of 1.8 mg/kg of antipyrine, the drug disappeared monoexponentially from plasma and salim for at least 51 hr, and the correlation between plasma half‐life and saliva half‐life was r = 0.97 (p < 0.001) in the 6 subjects. Excellent agreement was also observed between half‐lives after the high and low doses of antipyrine (r = 0.99, p < 0.001 for plasma and r = 0.98, p < 0.001 for saliva).