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Experimental perfusion system utilizing the rabbit Vx‐2 carcinoma: II. Correlative studies of in vitro sterilization of Vx‐2 cells with results of perfusions in rabbits and in patients
Author(s) -
Mori Shyunichi,
Clarkson Bayard,
O'Connor Annabel,
Lawrence Waiter
Publication year - 1962
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1002/cpt196234447
Subject(s) - in vitro , mitomycin c , methotrexate , pharmacology , cyclophosphamide , perfusion , chemistry , nitrogen mustard , in vivo , hela , chemotherapy , medicine , biology , immunology , surgery , biochemistry , microbiology and biotechnology
The effect of exposure to six chemotherapeutic agents incubated with Vx‐2 carcinoma cells in vitro has been determined by injecting cells into rabbits. 5x‐Fluoro‐2'‐deoxyuridine, methotrexate, and cyclophosphamide failed to cause sterilization of Vx‐2 cells in vitro and had little influence on subsequent tumor growth. HN2, actinomycin D, and mitomycin C all produced sterilization of Vx‐2 cells during in vitro incubation. The minimal sterilizing concentration of each of these drugs was established. The results of the in vitro tests were compared with the plasma concentration in the perfusion circuit during experimental rabbit hind limb perfusions as well as during pelvic perfusions in patients using these same drugs when suitable assay procedures were available (5‐fluoro‐2'‐deoxyuridine, methotrexate, actinomycin D, and mitomycin C). Except for cyclophosphamide, there was generally good correlation between the results of the in vitro tests with Vx‐2 cells and the therapeutic effect of perfusions.