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Serum MicroRNA‐150 Predicts Prognosis for Early‐Stage Non‐Small Cell Lung Cancer and Promotes Tumor Cell Proliferation by Targeting Tumor Suppressor Gene SRCIN1
Author(s) -
Zhang Liren,
Lin Jing,
Ye Yuanqing,
Oba Taro,
Gentile Emanuela,
Lian Jie,
Wang Jing,
Zhao Yang,
Gu Jian,
Wistuba Ignacio I.,
Roth Jack A.,
Ji Lin,
Wu Xifeng
Publication year - 2018
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1002/cpt.870
Subject(s) - microrna , gene knockdown , cancer research , lung cancer , cell growth , biology , untranslated region , suppressor , three prime untranslated region , cancer , gene , oncology , medicine , messenger rna , genetics
This integrative multistage study was aimed to identify circulating microRNAs (miRNAs) as prognostic biomarkers and investigate the treatment target for early‐stage non‐small cell lung cancer (NSCLC) patients. In stage I–II NSCLC patients, we screened and validated the miRNA ratio signatures predictive of prognosis in serum. In tumor, we found that the expression of miR‐150 in identified miRNA signatures was also associated with survival. Increased miR‐150 expression promoted NSCLC cell proliferation and migration and vice versa. Specific mRNA cleavage sites targeted by endogenous miR‐150 in 3′ untranslated region (UTR) of SRCIN1 was identified by utilizing our recently developed novel Stem‐Loop‐Array reverse‐transcription polymerase chain reaction (SLA‐RT‐PCR) assay. The blocking action of miR‐150 resulted in repressed NSCLC cell growth in vitro and knockdown of miR‐150 caused substantial tumor volume reduction in vivo . Our findings suggest that miR‐150 binding on specific recognition sites in 3′ UTR of tumor suppressor gene SRCIN1 present a potential therapeutic target for NSCLC.