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Interindividual and Regional Variability in Drug Transporter Abundance at the Human Blood–Brain Barrier Measured by Quantitative Targeted Proteomics
Author(s) -
Billington Sarah,
Salphati Laurent,
Hop Cornelis E. C. A.,
Chu Xiaoyan,
Evers Raymond,
Burdette Doug,
Rowbottom Christopher,
Lai Yurong,
Xiao Guangqing,
Humphreys W. Griffith,
Nguyen Tot Bui,
Prasad Bhagwat,
Unadkat Jashvant D.
Publication year - 2019
Publication title -
clinical pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.941
H-Index - 188
eISSN - 1532-6535
pISSN - 0009-9236
DOI - 10.1002/cpt.1373
Subject(s) - transporter , organic anion transporter 1 , blood–brain barrier , organic anion transporting polypeptide , p glycoprotein , glucose transporter , biology , organic cation transport proteins , atp binding cassette transporter , abcg2 , pharmacology , chemistry , drug resistance , biochemistry , endocrinology , multiple drug resistance , gene , central nervous system , genetics , insulin
For in vitro to in vivo extrapolation ( IVIVE ) of brain distribution of drugs that are transported at the human blood–brain barrier ( BBB ), it is important to quantify the interindividual and regional variability of drug transporter abundance at this barrier. Therefore, using quantitative targeted proteomics, we compared the abundance of adenosine triphosphate–binding cassette and solute carrier transporters in brain microvascular endothelial cells ( BMEC s) isolated from postmortem specimens of two matched brain regions, the occipital (Brodmann Area ( BA )17) and parietal ( BA 39) lobe, from 30 adults. Of the quantifiable transporters, the abundance ranked: glucose transporter (GLUT)1 > breast cancer resistance protein > P‐glycoprotein (P‐gp) > equilibrative nucleoside transporter ( ENT )1 > organic anion‐transporting polypeptide ( OATP )2B1. The abundance of multidrug resistance protein 1/2/3/4, OATP 1A2, organic anion transporter ( OAT )3, organic cation transporter ( OCT )1/2, OCTN 1/2, or ENT 2 was below the limit of quantification. Transporter abundance per gram of tissue (scaled using GLUT 1 abundance in BMEC vs. brain homogenate) in BA 17 was 30–42% higher than BA 39. The interindividual variability in transporter abundance (percentage of coefficient of variation (% CV )) was 35–57% ( BA 17) and 27–46% ( BA 39). These data can be used in proteomics‐informed bottom‐up IVIVE to predict human brain drug distribution.