Open AccessGenerating a Cost‐Effective, Weekend‐Free Chemically Defined Human Induced Pluripotent Stem Cell (hiPSC) Culture Medium
Author(s) -
Fonoudi Hananeh,
LyraLeite Davi M.,
Javed Hoor A.,
Burridge Paul W.
Publication year - 2020
Publication title -
current protocols in stem cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.658
H-Index - 28
eISSN - 1938-8969
pISSN - 1941-7322
DOI - 10.1002/cpsc.110
Subject(s) - human induced pluripotent stem cells , induced pluripotent stem cell , reprogramming , matrigel , chemically defined medium , biology , microbiology and biotechnology , cell culture , stem cell , cell , embryonic stem cell , in vitro , biochemistry , genetics , gene
We have previously developed a cost‐effective chemically defined medium formula for weekend‐free culture of human induced pluripotent stem cells (hiPSCs), costing ∼3% of the price of commercial medium. This medium, which we termed B8, is specifically optimized for robust and fast growth of hiPSCs and for a weekend‐free medium change regimen. We demonstrated that this medium is suitable for reprogramming of somatic cells into hiPSCs and for differentiation into a variety of lineages. Here, we provide a protocol for simple generation of the most cost‐effective variant of this medium, along with a protocol for making Matrigel‐coated plates and culturing, passaging, cryopreserving, and thawing hiPSCs. © 2020 Wiley Periodicals LLC. Basic Protocol 1 : Preparation of a highly optimized, robust, and cost‐effective human induced pluripotent stem cell culture medium Basic Protocol 2 : Weekend‐free maintenance and passaging of human induced pluripotent stem cells in B8 medium
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