Open AccessChronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound
Author(s) -
Kılıç Kıvılcım,
Tang Jianbo,
Erdener Ş. Evren,
Sunil Smrithi,
Giblin John T.,
Lee Blaire S.,
Postnov Dmitry D.,
Chen Anderson,
Boas David A.
Publication year - 2020
Publication title -
current protocols in neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 40
eISSN - 1934-8576
pISSN - 1934-8584
DOI - 10.1002/cpns.98
Subject(s) - neurovascular bundle , neuroimaging , medicine , neuroscience , protocol (science) , ultrasound , optical imaging , computer science , radiology , psychology , surgery , pathology , physics , alternative medicine , optics
Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC. Basic Protocol 1 : Surgical preparation of mouse chronic cranial windows using polymethylpentene Basic Protocol 2 : Imaging of mice with chronic cranial windows
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