Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis | Zendy

Chandrasekaran Arun Richard | Zendy; Halvorsen Ken | Zendy

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Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis

Author(s) -

Chandrasekaran Arun Richard,

Halvorsen Ken

Publication year - 2020

Publication title -

current protocols in nucleic acid chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.306

H-Index - 17

eISSN - 1934-9289

pISSN - 1934-9270

DOI - 10.1002/cpnc.115

Subject(s) - nuclease , dna , degradation (telecommunications) , biocompatibility , chemistry , gel electrophoresis , nanostructure , nanotechnology , computational biology , materials science , computer science , biology , biochemistry , telecommunications , organic chemistry

Custom‐built DNA nanostructures are now used in applications such as biosensing, molecular computation, biomolecular analysis, and drug delivery. While the functionality and biocompatibility of DNA makes DNA nanostructures useful in such applications, the field faces a challenge in making biostable DNA nanostructures. Being a natural material, DNA is most suited for biological applications, but is also easily degraded by nucleases. Several methods have been employed to study the nuclease degradation rates and enhancement of nuclease resistance. This protocol describes the use of gel electrophoresis to analyze the extent of nuclease degradation of DNA nanostructures and to report degradation times, kinetics of nuclease digestion, and evaluation of biostability enhancement factors. © 2020 Wiley Periodicals LLC. Basic Protocol : Timed analysis of nuclease degradation of DNA nanostructures Support Protocol : Calculating biostability enhancement factors

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