Open AccessImmunoelectron Microscopy of Viral Antigens
Author(s) -
Gulati Neetu M.,
Torian Udana,
Gallagher John R.,
Harris Audray K.
Publication year - 2019
Publication title -
current protocols in microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.813
H-Index - 35
eISSN - 1934-8533
pISSN - 1934-8525
DOI - 10.1002/cpmc.86
Subject(s) - immunoelectron microscopy , immunogold labelling , biology , virology , antigen , virus , negative stain , tetraspanin , electron microscope , antibody , immunology , genetics , cell , physics , optics
Immunoelectron microscopy is a powerful technique for identifying viral antigens and determining their structural localization and organization within vaccines and viruses. While traditional negative staining transmission electron microscopy provides structural information, identity of components within a sample may be confounding. Immunoelectron microscopy allows for identification and visualization of antigens and their relative positions within a particulate sample. This allows for simple qualitative analysis of samples including whole virus, viral components, and viral‐like particles. This article describes methods for immunogold labeling of viral antigens in a liquid suspension, with examples of immunogold‐labeled influenza virus glycoproteins, and also discusses the important considerations for sample preparation and determination of morphologies. Together, these methods allow for understanding the antigenic makeup of viral particulate samples, which have important implications for molecular virology and vaccine development. © 2019 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.