An In Vitro Microneutralization Assay for SARS‐CoV‐2 Serology and Drug Screening | Zendy

Amanat Fatima | Zendy; White Kris M. | Zendy; Miorin Lisa | Zendy; Strohmeier Shirin | Zendy; McMahon Meagan | Zendy; Meade Philip | Zendy; Liu WenChun | Zendy; Albrecht Randy A. | Zendy; Simon Viviana | Zendy; MartinezSobrido Luis | Zendy; Moran Thomas | Zendy; GarcíaSastre Adolfo | Zendy; Krammer Florian | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An In Vitro Microneutralization Assay for SARS‐CoV‐2 Serology and Drug Screening

Author(s) -

Amanat Fatima,

White Kris M.,

Miorin Lisa,

Strohmeier Shirin,

McMahon Meagan,

Meade Philip,

Liu WenChun,

Albrecht Randy A.,

Simon Viviana,

MartinezSobrido Luis,

Moran Thomas,

GarcíaSastre Adolfo,

Krammer Florian

Publication year - 2020

Publication title -

current protocols in microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.813

H-Index - 35

eISSN - 1934-8533

pISSN - 1934-8525

DOI - 10.1002/cpmc.108

Subject(s) - virology , serology , antibody , in vitro , antiviral drug , biology , drug , virus , covid-19 , coronavirus , immunology , pharmacology , medicine , infectious disease (medical specialty) , pathology , biochemistry , disease

The severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS‐CoV‐2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS‐CoV‐2 in vitro. © 2020 Wiley Periodicals LLC. Basic Protocol 1 : Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2 : Screening of anti‐SARS‐CoV‐2 compounds in vitro Support Protocol : SARS‐CoV‐2 propagation

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research