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Singlet Oxygen Oxidation of the Radical Cations of 8‐Oxo‐2′‐deoxyguanosine and Its 9‐Methyl Analogue: Dynamics, Potential Energy Surface, and Products Mediated by C5‐O 2 ‐Addition
Author(s) -
Moe May Myat,
Tsai Midas,
Liu Jianbo
Publication year - 2021
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.202100238
Subject(s) - chemistry , singlet oxygen , photochemistry , singlet state , deoxyguanosine , reaction mechanism , radical , potential energy surface , exothermic reaction , energetics , molecular dynamics , kinetics , molecule , computational chemistry , oxygen , adduct , organic chemistry , excited state , catalysis , physics , quantum mechanics , nuclear physics , ecology , biology
8‐Oxo‐2′‐deoxyguanosine (OG) is the most common DNA lesion. Notably, OG becomes more susceptible to oxidative damage than the undamaged nucleoside, forming mutagenic products in vivo. Herein the reactions of singlet O 2 with the radical cations of 8‐oxo‐2′‐deoxyguanosine (OG .+ ) and 9‐methyl‐8‐oxoguanine (9MOG .+ ) were investigated using ion‐molecule scattering mass spectrometry, from which barrierless, exothermic O 2 ‐addition products were detected for both reaction systems. Corroborated by static reaction potential energy surface constructed using multi‐reference CASPT2 theory and molecular dynamics simulated in the presence of the reactants′ kinetic and internal energies, the C5‐terminal O 2 ‐addition was pinpointed as the most probable reaction pathway. By elucidating the reaction mechanism, kinetics and dynamics, and reaction products and energetics, this work constitutes the first report unraveling the synergetic damage of OG by ionizing radiation and singlet O 2 .