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Doxorubicin‐Conjugated Iron Oxide Nanoparticles: Surface Engineering and Biomedical Investigation
Author(s) -
Oleksa Viktoriia,
Macková Hana,
Patsula Vitalii,
Dydowiczová Aneta,
Janoušková Olga,
Horák Daniel
Publication year - 2020
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.202000360
Subject(s) - conjugated system , nanoparticle , doxorubicin , carbodiimide , iron oxide nanoparticles , copolymer , polymer , chemistry , magnetic nanoparticles , polymer chemistry , combinatorial chemistry , materials science , organic chemistry , nanotechnology , chemotherapy , surgery , medicine
Development of therapeutic systems to treat glioblastoma, the most common and aggressive brain tumor, belongs to priority tasks in cancer research. We have synthesized colloidally stable magnetic nanoparticles ( D h =336 nm) coated with doxorubicin (Dox) conjugated copolymers of N,N ‐dimethylacrylamide and either N ‐acryloylglycine methyl ester or N ‐acryloylmethyl 6‐aminohexanoate. The terminal carboxyl groups of the copolymers were reacted with alendronate by carbodiimide formation. Methyl ester groups were then transferred to hydrazides for binding Dox by a hydrolytically labile hydrazone bond. The polymers were subsequently bound on the magnetic nanoparticles through bisphosphonate terminal groups. Finally, the anticancer effect of the Dox‐conjugated particles was investigated using the U‐87 glioblastoma cell line in terms of particle internalization and cell viability, which decreased to almost zero at a concentration of 100 μg of particles per ml. These results confirmed that poly( N,N ‐dimethylacrylamide)‐coated magnetic nanoparticles can serve as a solid support for Dox delivery to glioblastoma cells.