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Rapid Transmembrane Transport of DNA Nanostructures by Chemically Anchoring Artificial Receptors on Cell Membranes
Author(s) -
Li Min,
Liu Jiangbo,
Deng Mengying,
Ge Zhilei,
Afshan Noshin,
Zuo Xiaolei,
Li Qian
Publication year - 2019
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201900025
Subject(s) - endocytosis , transmembrane protein , membrane , chemistry , biophysics , reagent , cell membrane , transfection , membrane transport , transmembrane domain , receptor , biochemistry , biology , gene , organic chemistry
Highly efficient transmembrane transport of exogenous reagents into cells is of vital importance for developing drug‐delivery systems. Conventionally, transmembrane transport of exogenous reagents was accomplished with the assistance of transfection agents or other artificial means. However, the high toxicity and low transport efficiency of current delivery techniques still remain to be solved. In this work, by anchoring artificial receptors onto cell membranes with a mild chemical reaction, we demonstrated that the exogenous reagent framework nucleic acids, namely tetrahedral DNA nanostructures (TDN) can bind onto cell membranes effectively by the hybridization between single‐stranded DNA (ssDNA) and pendant ssDNA of TDN. The transport rate was greatly enhanced, with the endocytosis time could be as fast as 0.5 h. Furthermore, the transport quantity was prominently improved, with around 30 % of TDN endocytosed within 4 h. Owing to its rapid transmembrane transport speed and improved endocytosis quantity, this artificial‐receptor‐mediated transmembrane transport is a promising tool for achieving rapid and highly efficient transmembrane transport of exogenous reagents.