Premium
Amperometric Detection of dsDNA using an Acridine‐Orange‐Modified Glucose Oxidase
Author(s) -
Jambrec Daliborka,
Lammers Klaus,
Bobrowski Tim,
Pöller Sascha,
Schuhmann Wolfgang,
Ruff Adrian
Publication year - 2017
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201700373
Subject(s) - acridine orange , glucose oxidase , chemistry , dna , covalent bond , orange (colour) , biocatalysis , amperometry , combinatorial chemistry , biochemistry , biosensor , stereochemistry , organic chemistry , electrochemistry , reaction mechanism , catalysis , electrode , apoptosis , food science
Invited for this month's cover is the group of Prof. Dr. Wolfgang Schuhmann, Dr. Daliborka Jambrec and Dr. Adrian Ruff at Ruhr‐Universität in Bochum, Germany. The cover picture shows a novel procedure for the preferential post‐hybridization labeling of double‐stranded DNA based on the intercalating compound acridine orange, which was covalently bound to glucose oxidase. Labeling with a highly active biocatalyst allows for a simple and sequence‐independent amplification of the signal proportional to the amount of hybridized DNA that may be coupled with other amplification strategies. Read the full text of the article at 10.1002/cplu.201700279.