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Amperometric Detection of dsDNA Using an Acridine‐Orange‐Modified Glucose Oxidase
Author(s) -
Jambrec Daliborka,
Lammers Klaus,
Bobrowski Tim,
Pöller Sascha,
Schuhmann Wolfgang,
Ruff Adrian
Publication year - 2017
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201700279
Subject(s) - acridine orange , glucose oxidase , intercalation (chemistry) , chemistry , dna , biosensor , amperometry , combinatorial chemistry , biochemistry , hybridization probe , electrode , organic chemistry , electrochemistry , apoptosis
In the present “genomic era” and in the developing world of DNA chips, DNA detection based on intercalation of specific molecules is of particular interest because the detection process is largely independent of the sequence of the target DNA. In this work, an acridine‐orange‐based intercalation compound, which was tethered to deglycosylated glucose oxidase was synthesized ad hoc and investigated for its ability to interact with dsDNA. Amperometric detection of DNA hybridization was achieved by signal amplification based on the catalytic oxidation of glucose by DNA‐bound glucose oxidase. A clear distinction between dsDNA and ssDNA was achieved by careful design of a DNA‐modified electrode surface and prevention of nonspecific adsorption of the acridine‐orange‐modified enzyme by implementing a potential‐assisted immobilization method.