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A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina
Author(s) -
Pla Luís,
XifréPérez Elisabet,
Ribes Àngela,
Aznar Elena,
Marcos M. Dolores,
Marsal Lluís F.,
MartínezMáñez Ramón,
Sancenón Félix
Publication year - 2017
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201600651
Subject(s) - nanoporous , genomic dna , detection limit , mycoplasma , chemistry , dna , rhodamine b , rhodamine , anode , bacteria , fluorescence , microbiology and biotechnology , biophysics , nanotechnology , chromatography , biochemistry , genetics , biology , materials science , physics , electrode , organic chemistry , photocatalysis , quantum mechanics , catalysis
A nanoporous anodic alumina (NAA)‐based sensor system for the detection of Mycoplasma was developed through the implementation of “molecular gates” selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies⋅μL −1 and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.