Identification of Cellular MicroRNA Coupling Strand Displacement Polymerization and Nicking‐Endonuclease‐Based Cleavage | Zendy

Miao Peng | Zendy; Tang Yuguo | Zendy; Zhang Qi | Zendy; Bo Bing | Zendy; Wang Jue | Zendy

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Identification of Cellular MicroRNA Coupling Strand Displacement Polymerization and Nicking‐Endonuclease‐Based Cleavage

Author(s) -

Miao Peng,

Tang Yuguo,

Zhang Qi,

Bo Bing,

Wang Jue

Publication year - 2015

Publication title -

chempluschem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.801

H-Index - 61

ISSN - 2192-6506

DOI - 10.1002/cplu.201500249

Subject(s) - endonuclease , cleavage (geology) , microrna , lysis , chemistry , biophysics , multiple displacement amplification , polymerization , detection limit , dna , computational biology , materials science , biology , polymer , biochemistry , gene , chromatography , polymerase chain reaction , organic chemistry , fracture (geology) , dna extraction , composite material

A target induced recycling amplification method for the detection of cellular microRNA is reported based on strand displacement polymerization and nicking endonuclease mediated cleavage. Moreover, by utilizing silver nanoparticle‐based solid‐state Ag/AgCl reaction, ultrahigh sensitivity is achieved with powerful discriminating towards the identification of target microRNA. The limit of detection is as low as 70 a m . This method also allows the detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA expression profiling and disease diagnosis.

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