Premium
Identification of Cellular MicroRNA Coupling Strand Displacement Polymerization and Nicking‐Endonuclease‐Based Cleavage
Author(s) -
Miao Peng,
Tang Yuguo,
Zhang Qi,
Bo Bing,
Wang Jue
Publication year - 2015
Publication title -
chempluschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.801
H-Index - 61
ISSN - 2192-6506
DOI - 10.1002/cplu.201500249
Subject(s) - endonuclease , cleavage (geology) , microrna , lysis , chemistry , biophysics , multiple displacement amplification , polymerization , detection limit , dna , computational biology , materials science , biology , polymer , biochemistry , gene , chromatography , polymerase chain reaction , organic chemistry , fracture (geology) , dna extraction , composite material
A target induced recycling amplification method for the detection of cellular microRNA is reported based on strand displacement polymerization and nicking endonuclease mediated cleavage. Moreover, by utilizing silver nanoparticle‐based solid‐state Ag/AgCl reaction, ultrahigh sensitivity is achieved with powerful discriminating towards the identification of target microRNA. The limit of detection is as low as 70 a m . This method also allows the detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA expression profiling and disease diagnosis.